IUBio

PCR mutagenesis

D. KIM dkim at NMSU.Edu
Wed Mar 31 00:44:48 EST 1999


Mutagenesis by nucleotide misincorporation is only one approach to PCR
mutagenesis.  Another method is called "DNA Shuffling Mutagenesis", and is
described in papers by W. Stemmer.  I do not have specific citations on
hand, but a search of Medline may be able to come up with something.  The
concept is different from point mutagenesis by misincorporation, and is
reported to be more powerful.

Daniel Kim
 (p.s.  never tried it myself)



More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net