KI and DNA purification

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Mar 30 05:45:25 EST 1999


In article <bpmurray*STUFFER*-2903992052080001 at macmac-2.ucsf.edu>,
Bernard P. Murray, PhD <bpmurray*STUFFER*@socrates.ucsf.edu> writes
>> I have inherited a protocol for purifying DNA from agarose gels with
>> Promega's AgarACE enzyme.  The protocol I have calls for melting the gel
>> slices in 5.2M KI (final conc ~1.7M in ~300 ul total volume) before adding
>> the AgarACE.
>
>[snip]
>
>> Thanks,
>> Jennifer
>
>Is that really KI?  I thought most protocols user periodate
>(KIO4) or perchlorate for dissolution of agarose.  Sorry to
>question you, its just that the idea of using KI is new to me.

Don't know about KI but 6M NaI with 5g/l Na sulphite works fine. Store
in the dark or an opaque bottle etc. If it is stored in the light it
will go yellow. 

Duncan


-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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