KI and DNA purification
krasel at wpxx02.toxi.uni-wuerzburg.de
Tue Mar 30 04:21:41 EST 1999
Bernard P. Murray, PhD <bpmurray*STUFFERemail@example.com> wrote:
> In article <7dp5pu$7ht$1 at nnrp1.dejanews.com>, jag100 at snethen.com wrote:
>> I have inherited a protocol for purifying DNA from agarose gels with
>> Promega's AgarACE enzyme. The protocol I have calls for melting the gel
>> slices in 5.2M KI (final conc ~1.7M in ~300 ul total volume) before adding
>> the AgarACE.
> Is that really KI? I thought most protocols user periodate
> (KIO4) or perchlorate for dissolution of agarose.
I was told that GeneClean uses KI for dissolving agarose.
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
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