KI and DNA purification

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Tue Mar 30 04:21:41 EST 1999


Bernard P. Murray, PhD <bpmurray*STUFFER*@socrates.ucsf.edu> wrote:
> In article <7dp5pu$7ht$1 at nnrp1.dejanews.com>, jag100 at snethen.com wrote:
>> I have inherited a protocol for purifying DNA from agarose gels with
>> Promega's AgarACE enzyme.  The protocol I have calls for melting the gel
>> slices in 5.2M KI (final conc ~1.7M in ~300 ul total volume) before adding
>> the AgarACE.
> 
> Is that really KI?  I thought most protocols user periodate
> (KIO4) or perchlorate for dissolution of agarose.

I was told that GeneClean uses KI for dissolving agarose.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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