IUBio

PCR mutagenesis

toufik toufik.abbas-terki at cellbio.unige.ch
Tue Mar 30 11:53:57 EST 1999


Hye,

Does any one have a protocol for random mutagenesis by PCR?
I tried with 1mM of dCTP/dTTP/dGTP and 0.2mM of dATP, but  my
amplification didn't work. (the standart amplification works well!).

Thank you for your help


Toufik




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