KI and DNA purification

Clemens Suter-Crazzolara c.suter-crazzolara at dkfz-heidelberg.de
Wed Mar 31 04:25:35 EST 1999

> > > I have inherited a protocol for purifying DNA from agarose gels with
> > > Promega's AgarACE enzyme.  The protocol I have calls for melting the gel
> > > slices in 5.2M KI (final conc ~1.7M in ~300 ul total volume) before adding
> > > the AgarACE.

why purify ? if the frags are smaller than 1500 bp, cut the agarose from the gel,
put it into an eppendorf with a hole and a plug (f.i. galsswool) and spin for one
minute. Your frags are ready to be ligated.


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