Integration or not

Michael Wu mwu at SMTP.MCIS.UCHICAGO.EDU
Wed Mar 31 17:23:17 EST 1999


I have human LTC-IC colonies that are fluorescent (after transfection).
Is there a practical method to prove that the GFP gene is INTEGRATED
into the cell chromosome? The plasmid used was pEGFP-1 with no origin of
replication in mammalian cells. I have thought about FISH, Reverse PCR,
and Souther hybridization. All have major disadvantages (there are only
about 50 cells to start with).
Is there any commercial kit available for this?
Any sugestion is highly appreciated.

Thanks

Michael, University of Chicago
email: mwu at mcis.bsd.uchicago.edu




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