Acrylamide

Rich Dudley rdudley+ at pitt.edu
Wed Mar 31 16:34:01 EST 1999


Matthias Maiwald wrote:

> Hi all,
>
> in my old lab in Germany we used to purchase a ready-mixed Acrylamide/Bis
> solution from a small chemical company which we used for protein
> electrophoresis or for DNA sequencing. The solution was stabilized by
> argon gas to prevent it from deteriorating. Does anyone have an American
> company or distributor in mind which sells something alike?
>
> Cheers, Matthias.
>
> Matthias Maiwald, MD
> Department of Microbiology and Immunology
> Stanford University School of Medicine
> E-MAIL: maiwald at cmgm.stanford.edu

Try Amresco's PAGE-Plus (www.amresco-inc.com).  I use it for SDS-PAGE as well
as sequencing gels.  This is the acrylamide ABI recommends for their
automated sequencers.  Unfortunately, they don't send a cheat-sheet for
SDS-PAGE like they do for DNA gels.  I'm hounding them to do so, but in the
meantime, if you would need the amounts to mix, let me know.

Good luck!

rich

--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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