Matthias Maiwald wrote:
> Hi all,
>> in my old lab in Germany we used to purchase a ready-mixed Acrylamide/Bis
> solution from a small chemical company which we used for protein
> electrophoresis or for DNA sequencing. The solution was stabilized by
> argon gas to prevent it from deteriorating. Does anyone have an American
> company or distributor in mind which sells something alike?
>> Cheers, Matthias.
>> Matthias Maiwald, MD
> Department of Microbiology and Immunology
> Stanford University School of Medicine
> E-MAIL: maiwald at cmgm.stanford.edu
Try Amresco's PAGE-Plus (www.amresco-inc.com). I use it for SDS-PAGE as well
as sequencing gels. This is the acrylamide ABI recommends for their
automated sequencers. Unfortunately, they don't send a cheat-sheet for
SDS-PAGE like they do for DNA gels. I'm hounding them to do so, but in the
meantime, if you would need the amounts to mix, let me know.
--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
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