IUBio

PCR in palindrome...

laurent houzet lhouzet at alize.ulb.ac.be
Wed Mar 31 12:03:50 EST 1999


Hi everybody,
Does anybody has an idea of how to amplify a sequence located in a
palindrome of about 2.5 pb...I tried without special care and I didn't
success...any idea, reference or experience for this kind of PCR
amplification, with this kind of structure which could complicate the
annealing step, would be welcome...

Thanks a lot

Laurent




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