IUBio

Questions about clean up of PCR product

Sameez Hasan shasan at vetbio.unizh.ch
Wed Mar 31 10:09:50 EST 1999


In article <7dt7ek$rr02 at hkusud.hku.hk>, "Wilson" <netson at hotmail.com> wrote:

> Dear all,
> I would like to know the effective and economic way(s) to clean up PCR
> product.  I always use the following procedures: precipitate PCR product,
> then have a agarose gel electrophoresis, cut out the target DNA band and
> retrieve DNA by GeneClean.  Is there any other way (such as special
> precipitation methods) to remove impurities such as primers and dNTPs
> effectively?
> 
> Thank you very much
> 
> Wilson
Hi
What we're commonly using is Qiagen's PCR-purification Kit (it should be
the same as the gel extraction kit). It's quick, you have a good yield,
and it's very reliable.
Sam

-- 
Sameez Hasan
Institut fur Veterinarbiochemie               Tel: (41-1)-635-54-75
Universitat Zurich-Irchel                     Fax: (41-1)-635-68-40
Winterthurerstrasse 190
CH-8057 Zurich
Switzerland                             <http://www.unizh.ch/vetbio>



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