I will construct random expression cDNA library from a viral genome (RNA and
the size is about 12.3kb). As this is a first time for me to perform the
experiment, I would like to seek advice from the experienced expert that
have performed the construction before.
My strategy is to purify viral RNA first, then reverse transcribe to
first-stranded cDNA by random hexamer. The next step is second-stranded
synthesis. What is the effective (or best) way to perform the synthesis for
further cloning into expression plasmid vector?
Thank you very much