IUBio

Questions about clean up of PCR product

Wilson netson at hotmail.com
Wed Mar 31 16:19:00 EST 1999


Dear all,
I would like to know the effective and economic way(s) to clean up PCR
product.  I always use the following procedures: precipitate PCR product,
then have a agarose gel electrophoresis, cut out the target DNA band and
retrieve DNA by GeneClean.  Is there any other way (such as special
precipitation methods) to remove impurities such as primers and dNTPs
effectively?

Thank you very much

Wilson





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