Isolation of intact mitochondria from cultured human tumor cells

Geoff Birrell gbirrell at
Mon May 3 17:29:41 EST 1999

I am after a protocol to isolate mitochondria. I have tried homogenizing
in 0.25 M sucrose in tight fit glass mortar and pestle  but get high
contamination from lipid membranes after spinning at 1500 g. I would
appreciate advice from experienced mitochondriacs.

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