Richard P. Grant
r_grant at see.sig.for.address
Tue May 11 07:45:37 EST 1999
In article <007801be9b16$a63305a0$8c01a8c0 at VS_lab.tn.nic.in>,
jakku at mrna.tn.nic.in ("R. Jayakumar") wrote:
> CAn anyone tell me why ethanol is normally used to precipitate DNA after
>it has been cooled below 0 Celsius. But I have come across atleast one
>protocol where ethanol has been used at room temperature for DNA
>precipitation. Why this temperature specifications for ethanol, while
>isopropanol is used at room temperature?
Heh, news to me. I always use EtOH at RT . . .
Using IPA below 0C is a bad idea because you increase the risk of bringing
Richard P. Grant MA DPhil | rgrant at cmtech.co.uk
work: www.cmtech.co.uk | home: www.avnet.co.uk/adastra
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