CytoTrap yeast-two-hybrid

Patrick Sieber psieber at ds.unizh.ch
Wed May 12 09:56:08 EST 1999


Hi
I am planning to do a yeast-two-hybrid screen using the Cyto-Trap kit
(Stratagene).
Has anybody ever used it? What experiences did you make?
My bait protein is from Arabidopsis thaliana and has no homology to
anything in the database. It could be a transcription factor and it is
located in the nucleus (GUS).
Would you recommend using this kit?
Thanks for help
Patrick




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