rest. digest on kit extracted vs phenol extracted DNA
Richard P. Grant
r_grant at see.sig.for.address
Fri May 14 04:30:08 EST 1999
In article <7heurs$87s_001 at leeds.ac.uk>, BGYCSW at leeds.ac.uk (C.S. Wilding)
>Thanks for the message. The protocol is not very forthcoming with recipes
>but 1 of the "buffers" contains "a chaotropic salt" and another contains
>sodium azide. I am not sure what the column is but presume it is just some
>kind of filter to retain the chaotropic salt with bound DNA.
Sounds like silica-binding then (that's what the chaotroph is for).
The azide is just biocidal and shouldn't affect downstream stuff.
 Ow! Nasty preposition! :-)
Richard P. Grant MA DPhil | rgrant at cmtech.co.uk
work: www.cmtech.co.uk | home: www.avnet.co.uk/adastra
-- I laugh in the face of danger. Then I hide until it passes --
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