rest. digest on kit extracted vs phenol extracted DNA

[Richard P. Grant]

In article <7heurs$87s_001 at leeds.ac.uk>, BGYCSW at leeds.ac.uk (C.S. Wilding)
wrote:

>Thanks for the message. The protocol is not very forthcoming with recipes 
>but 1 of the "buffers" contains "a chaotropic salt" and another contains 
>sodium azide. I am not sure what the column is but presume it is just some 
>kind of filter to retain the chaotropic salt with bound DNA.

Sounds like silica-binding then (that's what the chaotroph is for)[0].
The azide is just biocidal and shouldn't affect downstream stuff.

R

[0] Ow!  Nasty preposition!  :-)

-- 
Richard P. Grant MA DPhil     |             rgrant at cmtech.co.uk
work: www.cmtech.co.uk        |      home: www.avnet.co.uk/adastra
 -- I laugh in the face of danger. Then I hide until it passes --