Help with RevTet system from Clontech

jjj jjj at notme.com
Mon Nov 1 13:09:53 EST 1999


Hello everybody!!

We have been trying to use the RevTET system from Clontech for inducible
expression in 3T3-L1 without any success.

We have tried both serial and simultaneous infection of the target
cells, with virus produced from both transiently transfected packing
cells and selected packing cells.
We have used Doxyciclin (0,5 and 1 µg/ml) for induction during 12, 24,
72 hours.

No expresion whatsoever!!!

Any feedback from successful AND unsuccessful attempts to use this
system will be very welcomed (preferably by E-mail).

Thanks!
Juan_Antonio.Contreras at medkem.lu.se





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