Yeast media and growth

[Frank O. Fackelmayer]

Younes Bounhar wrote:

> I am trying to express proteins in yeast using a the Gal1 promoter) and
> cannot afford to get raffinose (I tried glucose then galactose only and did
> not get any induction. A friend of mine suggested to use a mixture of
> 3%Glycerol and 2%Ethanol. this allows for a very nice induction but the
> cells grow very very slowly if at all (it takes over 72h to get an
> OD600~0.6). Does anybody know why or if there is another alternative?
>
> Younes
>
> --
> Be Yourself, no matter what they say

Hi Younes,
What about growing the cells in a medium they like to get biomass first, then
spin them down and concentrate, say tenfold, by resuspension in induction
medium.

just a thought,
Frank


Frank