Isolated nuclei

[Stephen Snowdy]

Does anyone out there do isolated nuclei work?  I was wondering:  when
you fractionate nuclei out of cell lysates, do cytoplasmic proteins
remain attached to the outside of the pore complexes?  Does anyone have
a protocol for isolating nuclei that leaves no question that all
proteins found were inside the nucleus and not just attached to the
outside at the NPC's?

Thank you