repeat and plasmid yield

Bongjune Yoon bjyoon at ix.netcom.com
Mon Nov 1 22:14:13 EST 1999


I am trying to clone a very GC rich repetitive DNA fragment. I have been
getting very low yield of plasmid DNA (sometimes with some smear like
contamination on the gel check). Does this have something to do with the
repeat sequences? Does anybody have similar experience?






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