FIX-II Phage library construction - Low yield ! Why ?

Frederik Boernke boernke at nospam.ipk-gatersleben.de
Tue Nov 2 16:17:19 EST 1999


dboehm wrote:
> 
[snip]


Hi,

unfortunately I don't have the solution for your particular problem
but I experienced one thing when using Stratagene's library construction
kits: In my hand it was absolutely crucial to use Stratagene's ligase to
get satisfactory results. When I used Boehringer's ligase to construct
a genomic library into ZAP-Express it didn't work at all, when I
switched
to Stratagene's ligase, which didn't come with the kit, everything was
perfect no matter what insert to vector ratio I used.
Hope this helps

Ricky


*******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
http://www.ipk-gatersleben.de




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