pcr from ligation

Guy Tremblay tremblgu at yahoo.com
Tue Nov 2 12:03:31 EST 1999


Mark Edward Bowen wrote:

> I want to ligate two dna fragments together an amplify the resultant
> product for subcloning.    Does anyone have any tips on getting this to
> work well.  Is it necessary to clean up the ligation before PCR?  Should
> I keep the ligation equimolar or doesn't it matter?  Will  self-
> ligation products products prove probelmatic?

http://blume.bch.umontreal.ca/genefusion.html





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