pKa of histidines in His-tag

Kresten kresten at
Wed Nov 3 09:55:13 EST 1999

Does anybody know whether the pKa values of Histidines in a His-tag
differs significantly from "normal" pKa values of Histidine?

If somebody has the pKa of a His residue in the middle of poly-His,
please let me know.

Thank you

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Kresten Lindorff Larsen, Dept. Yeast Genetics

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