Reporter for macrophage (J774) transfection

C Coward cc122 at mole.bio.cam.ac.uk
Wed Nov 3 10:57:30 EST 1999


Greetings,

I am investigating the ability of a novel compound to act as a
transfection aid for a macrophage cell line (J774A). Previously I have
observed that the pSV-b-gal plasmid (Promega) poorly expresses b-gal in
this cell line (or poorly transfects it). I am looking for a reporter
plasmid suitable for use in J774, preferably with a straightforward assay
method for the degree of transfection. Does anyone have any suggestions?

Cheers in advance.

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