Isolated nuclei

[redflag]

Good questions !

I isolated nuclei from myeloid TF-1 derived cells. It's difficult to obtain
good results. As there was not no doubt about the integrity of our nuclei,
my report finally presents results on whole cells...
I used classical and fluorescence microscopies to ascertain it was nuclei.
I have a protocol, I just have to find it again. I did this work for my
master degree internship in Toulouse, France. Contact me by email for
further info.
By the way, where are you ?

Seb

Sébastien Charpentier
Montréal, Canada.
scharpen at videotron.ca


Stephen Snowdy a écrit :

> Does anyone out there do isolated nuclei work?  I was wondering:  when
> you fractionate nuclei out of cell lysates, do cytoplasmic proteins
> remain attached to the outside of the pore complexes?  Does anyone have
> a protocol for isolating nuclei that leaves no question that all
> proteins found were inside the nucleus and not just attached to the
> outside at the NPC's?
>
> Thank you