Immunostaining of nuclear proteins

Stephen Snowdy stephen_snowdy at med.unc.edu
Fri Nov 5 00:07:45 EST 1999


Does anyone know if methanol does a good job of permeabilizing adherent
cells all the way down to the nucleus?  Are any special steps necessary
to immunostain epitopes inside the nucleus?
-- 
Stephen Snowdy
Department of Pharmacology
College of Medicine
University of North Carolina-Chapel Hill




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