Immunostaining of nuclear proteins

Stephen Snowdy stephen_snowdy at
Fri Nov 5 00:07:45 EST 1999

Does anyone know if methanol does a good job of permeabilizing adherent
cells all the way down to the nucleus?  Are any special steps necessary
to immunostain epitopes inside the nucleus?
Stephen Snowdy
Department of Pharmacology
College of Medicine
University of North Carolina-Chapel Hill

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