Immunostaining of nuclear proteins

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Fri Nov 5 09:49:34 EST 1999


In article <38226621.CB03C587 at med.unc.edu>, Stephen Snowdy
<stephen_snowdy at med.unc.edu> wrote:
> Does anyone know if methanol does a good job of permeabilizing
> adherent
> cells all the way down to the nucleus?  Are any special steps
> necessary
> to immunostain epitopes inside the nucleus?
> --
> Stephen Snowdy
> Department of Pharmacology
> College of Medicine
> University of North Carolina-Chapel Hill

Methanol fixation works for PCNA staining. Of course, depending on the
accessability of your particular epitope, YMMV.

Nick



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