DNA Methylation analysis - help please !!

rogier stugerNOstSPAM at cellbiology.uni-frankfurt.de.invalid
Fri Nov 5 13:00:13 EST 1999


bisulfite modification turns C into U but leaves methylated Cs alone.
so do bisulfite modification, then PCR, then sequence
(5-lane sequence: ACGT from the unmodified DNA, and a C or T lane from
the modified DNA)
Good luck,
Rogier Stuger
Dept MicFizz, Free U of A
rogier at biogate.com


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