how do I get rid of adapters / linkers after ligation to my cDNA
ralf.sigmund at mpihan.mpg.de
Sat Nov 6 13:04:20 EST 1999
I am trying to digest ds-cDNA with a four base cutter Restriction Enzyme.
Afterwards I ligate an adapter to both cutted ends.
I now want to PCR-Amplify my cDNA Fragment with Primers in the Adapter
This apperently fail because the PCR is completely dominated by
So I would like to get rid of theese 58 BP Adapter dimers.
However the amount of cDNA is very limited, so I fear to loose too much if I
run an agarose Gel...
Thanx for any hints
More information about the Methods