"Re: >High GC PCR"

Noula Pouroullis npouroullis at CSIR.CO.ZA
Mon Nov 8 03:44:21 EST 1999


I was able to amplify high GC organisms - (streptomycetes)- by adding 10% Acetamide to a normal PCR reaction- 
I would titrate the acetamide to find the optimum concentration for your application. It made all the difference to my project.

good luck

Myrsini Pouroullis






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