pNUT expression vector

Tom Vink tvink at xs4all.nl
Mon Nov 8 05:24:17 EST 1999


On Thu, 04 Nov 1999 17:09:19 -0500, Rich Dudley <rdudley+ at pitt.edu>
wrote:

>Has anyone ever heard of/used an expression vector called pNUT?  Can you
>point me to a reference or a source if you have?  Thanks!
>
>rich
>
>--
>Richard J. Dudley (rdudley+ at pitt.edu)
>Research Specialist V
>Cystic Fibrosis Research Center
>Dept. of Cell Biology and Physiology
>University of Pittsburgh
>http://www.cbp.pitt.edu
>---> search BIONET archives at http://www.bio.net <---
>
>

Yep, A mammalian expression vector with a mutant DHFR gene as
selection marker and a mouse MT promotor. It is from 1986 I believe
(look for Palmiter, Cell) but it works fine and I still use it. Works
best in combination with BHK cells but should be usable for any cell
type.

Greetings Tom





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