pNUT expression vector

Tom Vink tvink at
Mon Nov 8 05:24:17 EST 1999

On Thu, 04 Nov 1999 17:09:19 -0500, Rich Dudley <rdudley+ at>

>Has anyone ever heard of/used an expression vector called pNUT?  Can you
>point me to a reference or a source if you have?  Thanks!
>Richard J. Dudley (rdudley+ at
>Research Specialist V
>Cystic Fibrosis Research Center
>Dept. of Cell Biology and Physiology
>University of Pittsburgh
>---> search BIONET archives at <---

Yep, A mammalian expression vector with a mutant DHFR gene as
selection marker and a mouse MT promotor. It is from 1986 I believe
(look for Palmiter, Cell) but it works fine and I still use it. Works
best in combination with BHK cells but should be usable for any cell

Greetings Tom

More information about the Methods mailing list