>High GC PCR"

Don Walthers gene_jockey26 at yahoo.com
Mon Nov 8 19:19:33 EST 1999


Lowering the annealing temp and increasing the annealing time plus the
addition of 5% DMSO helps my GC-rich PCR (Myxococcus). My primers usually
have Tm's of about 85-90 degrees. I usually use 95 1', 50-55 2' and 72 1'/kb
expected product.

--
Don Walthers
B.S.Microbiology U of Illinois at UC, 1996
Ph.D. Microbiology U of Idaho, 2001?
gene_jockey26 at yahoo.com






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