Toxic beta-galactosidase substrate?
Dr. Hedley Carr
bmbthc at removethisbmb.leeds.ac.uk
Tue Nov 9 06:48:30 EST 1999
Does anyone know of a beta-galactosidase substrate that yields
a product toxic to E.coli only if it is cleaved ? (i.e: only if
the cells are expressing LacZ). I'm after a selection method which
will screen out all unwanted non-recombinants (i.e: LacZ intact).
School of Biochem. & Mol. Biol.,
University of Leeds.
Note: 'REMOVE' included in reply address to prevent spam.
More information about the Methods