TA cloning kit

John R. McQuiston zje8 at cdc.gov
Tue Nov 9 15:02:52 EST 1999

I think you'd be happy with Invitrogen's TOPO TA cloning kit.  We are.


In article <38284ced.0 at news.uni-ulm.de>, fwagner at medizin.uni-ulm.de says...
>For several years, we used the Amersham pMosBlue T-vector kit for TA
>cloning. It was very satisfying with normal Taq and with Boehringer
>high-fidelity PCR system. Now I had to learn that Amersham do no more
>produce this kit and instead sell a blunt-end cloning kit that they claim to
>be even superior.
>My questions:
>Is this blunt-end pMosBlue PCR cloning kit really comparable or even better
>to the pMosBlue T-vector kit (my insert size is about 500 to 5,000 bp)?
>Are there other companies selling _good_ T-vector kits?
>I would be happy for any recommendations based on personal experience.
>I thank you very much in advance

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