TA cloning kit
dmicklem at cmgm.nospam.invalid
Tue Nov 9 16:51:13 EST 1999
In article <38284ced.0 at news.uni-ulm.de>, Dr. med. Franz F. Wagner
<fwagner at medizin.uni-ulm.de> wrote:
>For several years, we used the Amersham pMosBlue T-vector kit for TA
>cloning. It was very satisfying with normal Taq and with Boehringer
>high-fidelity PCR system. Now I had to learn that Amersham do no more
>produce this kit and instead sell a blunt-end cloning kit that they claim to
>be even superior.
>Is this blunt-end pMosBlue PCR cloning kit really comparable or even better
>to the pMosBlue T-vector kit (my insert size is about 500 to 5,000 bp)?
>Are there other companies selling _good_ T-vector kits?
>I would be happy for any recommendations based on personal experience.
>I thank you very much in advance
Notwithstanding recent negative experiences reported on this newsgroup
(with rearranged clones/loss of polylinker sequences) I have found the
Invitrogen TOPO-TA vectors to work fantastically well.
They are kind of expensive as they insist on supplying competent cells
with the vector, but if you have a source of other cells you can make
the vector last at least 2-3 times as long by scaling down their
D.R. Micklem, Time flies like an arrow... Fruit flies like a banana.
Beckman Center, Email:dmicklem at cmgm.stanford.edu
Stanford University Phone: +1 (650) 723-6650
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