Re-equilibrating phenol

R. Jayakumar jakku at mrna.tn.nic.in
Wed Nov 10 01:35:15 EST 1999


That should to it OK.  But start with a lower molarity buffer and then after
two washes go onto the molarity you want.  Funny, but I though EDTA
precipitated at pHs below 8.0.  anyone correct me?
jakku

----- Original Message ----- 
From: <Guy.Oshiro at uchsc.edu>
To: <methods at hgmp.mrc.ac.uk>
Sent: Wednesday, November 10, 1999 3:08 AM
Subject: Re-equilibrating phenol


> Hi,
> 
> I have some Phenol that I bought pre-equilibrated in Tris and EDTA at a
> pH of 6.6. But I need the phenol equlibrated at pH 5.3 in 50 mM sodium
> acetate and 10 mM EDTA. Is there any problems in just re-equlibrating
> the phenol in the other buffer. I want to make sure that I don't have
> anything precipitating out.
> 
> Thanks for the help.
> 
> -Guy
> 
> 
> Sent via Deja.com http://www.deja.com/
> Before you buy.
> 

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