genomic DNA isolation from bacteria

Enteng & Sheila de Jesus boston at QUICKWEB.COM.PH
Wed Nov 10 03:47:21 EST 1999


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Dear all,

I am initiating to do an 16srDNA research with my undergrad students and =
due to some limitations, I wonder if anyone can share me a protocol with =
regards to a simple DNA  isolation from gram negative bacteria without =
the use of phenol & chloroform. =20

I appreciate any advice and assistance u can provide.

Ma. Sheila M. de Jesus
Dept. of Biological Sciences
College of Science
University of Santo Tomas
Philippines



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<DIV><FONT face=3D"Bell MT" size=3D2>Dear all,</FONT></DIV>
<DIV>&nbsp;</DIV>
<DIV><FONT face=3D"Bell MT" size=3D2>I am initiating to do an 16srDNA =
research with=20
my undergrad students and due to some limitations, I wonder if anyone =
can share=20
me a protocol with regards to a simple DNA&nbsp; isolation from gram =
negative=20
bacteria without the use of phenol &amp; chloroform.&nbsp; </FONT></DIV>
<DIV>&nbsp;</DIV>
<DIV><FONT face=3D"Bell MT" size=3D2>I appreciate any advice and =
assistance u can=20
provide.</FONT></DIV>
<DIV>&nbsp;</DIV>
<DIV><FONT face=3D"Bell MT" size=3D2>Ma. Sheila M. de Jesus</FONT></DIV>
<DIV><FONT face=3D"Bell MT" size=3D2>Dept. of Biological =
Sciences</FONT></DIV>
<DIV><FONT face=3D"Bell MT" size=3D2>College of Science</FONT></DIV>
<DIV><FONT face=3D"Bell MT" size=3D2>University of Santo =
Tomas</FONT></DIV>
<DIV><FONT face=3D"Bell MT" size=3D2>Philippines</FONT></DIV>
<DIV>&nbsp;</DIV>
<DIV>&nbsp;</DIV></BODY></HTML>

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