genomic DNA isolation from bacteria

Gerd  gerdn at
Wed Nov 10 09:32:20 EST 1999

>Dear all,
>I am initiating to do an 16srDNA research with my undergrad students and =
>due to some limitations, I wonder if anyone can share me a protocol with =
>regards to a simple DNA  isolation from gram negative bacteria without =
>the use of phenol & chloroform. =20

What about some genomic DNA preparation kit? (Qiagen has one, and probably
the other molbio companies also have something); if that's not an option - I
guess the limitations has to be specified.


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