TA cloning kit

Ruderion nospam~r.vandewetering at czzorlnm.azn.nl
Wed Nov 10 10:45:16 EST 1999


>Notwithstanding recent negative experiences reported on this newsgroup
>(with rearranged clones/loss of polylinker sequences) I have found the
>Invitrogen TOPO-TA vectors to work fantastically well.
>
>They are kind of expensive as they insist on supplying competent cells
>with the vector, but if you have  a source of other cells you can make
>the vector last at least 2-3 times as long by scaling down their
>recommended reaction.
>
We've succesfully scaled down 5 times, also by using their cells. Everything
works fine but plate everything in the end not just a little.

Rudi






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