cell line suggestion

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Wed Nov 10 16:21:09 EST 1999


In article <3829DE40.BB66C957 at ouhsc.edu>, "Kajetan H. Groicher"
<Kajetan-Groicher at ouhsc.edu> wrote:
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> Hello all-
> I am in need of a good all around human endothelial cell line for
> use in
> viral transformation studies, as well as studies of certain viral
> genes.  I have looked through some of the thousands at ATCC, but
> most
> have been derived from leukemias, etc.  I would prefer one that is
> "normal" (as normal as a culturable cell line can be).  The big
> restriction is that I am looking for an endothelial line.
> Thanks in advance.
> Kajetan

A lot of people use HUVECS (human umbilical vein endothelial cells) as
a "generic" endothelial cell. You can buy them from Clonetics, and some
people even isolate them themselves from cords (of course, you need
access to cords for that).

Nick



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