Multiple amino acid mutagenesis

Noam Harel hareln at mail.med.upenn.edu
Wed Nov 10 19:26:09 EST 1999


Hi,

I plan to mutate a stretch of up to 18 contiguous amino acids in a
600-residue protein. What would have the least predicted effect on
overall protein structure? mutating to all alanines, all glycines, a
mixture of both, or just deleting that stretch? 

Any suggestions would be muchly appreciated...

Noam Harel
University of Pennsylvania




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