Cloning of small DNA fragments

J Stevenson pcxjs at unix.ccc.nottingham.ac.uk
Thu Nov 11 06:33:13 EST 1999


HI

I'm having problems cloning synthetic DNA fragments (88-130 bp) into any
plasmid vector with EcoR1 and BamH1 restriction sites. I think it might be
that the enzyme digest isn't working because of the small size.
 
Does anyone have any experience successfully digesting/cloning fragments
of this size?
James
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James Stevenson
Department of Chemistry
University of Nottingham
University Park Nottingham
NG7 2RD

Tel: 0115 9514193
e-mail: pcxjs at unix.ccc.nottingham.ac.uk
www: http://www.nottingham.ac.uk/~pcxjs/thomas/
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