T-tail

[Jarmo Schrader]

In article <382A2582.1E68E202 at stromix.com>, sean at stromix.com wrote:

> Can someone give me a protocol for adding a 3' T overhang to a cut
> vector for TA cloning of PCR products.
> 

Kovalic et al. 1991, NAR 19:4560-4563

They inserted a short oligo into the NcoI site of Promegas pGEM-5Zf.
Cutting with XcmI results in the T overhangs. 

Contact me by e-mail if you want the sequence.

Jarmo

-- 
Jarmo Schrader     Umea, Sweden

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