Jarmo.Schrader.removethis at genfys.slu.se
Thu Nov 11 07:44:08 EST 1999
In article <382A2582.1E68E202 at stromix.com>, sean at stromix.com wrote:
> Can someone give me a protocol for adding a 3' T overhang to a cut
> vector for TA cloning of PCR products.
Kovalic et al. 1991, NAR 19:4560-4563
They inserted a short oligo into the NcoI site of Promegas pGEM-5Zf.
Cutting with XcmI results in the T overhangs.
Contact me by e-mail if you want the sequence.
Jarmo Schrader Umea, Sweden
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