Mini centrifuge filters for protein binding studies

J Stevenson pcxjs at unix.ccc.nottingham.ac.uk
Thu Nov 11 11:27:36 EST 1999


Dear Hugh

I use Pall Filtron Nanosep microconcentrators for protein sample
concentration. They can be spun down to zero retention volume if left long
enough, and come in a range of sizes and MW cutoffs

Their address is:

Pall Filtron Corporation
50 Bearfoot Road
Northborough
MA 01532
call (508) 393-1800
fax (5080 393-1874

*******************************************************************************
James Stevenson
Department of Chemistry
University of Nottingham
University Park Nottingham
NG7 2RD

Tel: 0115 9514193
e-mail: pcxjs at unix.ccc.nottingham.ac.uk
www: http://www.nottingham.ac.uk/~pcxjs/thomas/
*******************************************************************************


On 10 Nov 1999, Hugh Goldie wrote:

> I would like to use mini centrifuge filters for studies of small radioactive 
> ligands binding to a purified protein.  At present I have a pressure cell,
> with eight chambers, which uses nitrogen gas pressure to force samples 
> through 10,000 MW (Amicon TM10) filters; however, this is rather cumbersome 
> and slow.
> 
> Commercial filter units are available with 10,000 MW cut-offs; however, these
> are intended for _concentrating_ small protein samples.  They are constructed
> so that the solution being filtered will not all pass through the filter.  
> (They have a "retentate volume" of 10 microlitres.)  
> 
> What I need are minicentrifuge tubes with zero retentate volume (i.e. all
> the solution passes through the filter and the protein is left behind with
> bound radioactive ligand.)  The total volume of my binding reactions is 100 
> microlitres, so a 1.5 mL centrifuge tube would be a good size.  Does anyone 
> know where to obtain these?  I am trying to make some from various 1.5 mL
> and 0.5 mL tubes, but it is tricky.
> 
> --Hugh
> 
> --
> Hughes Goldie, Department of Microbiology and Immunology
> Saskatoon, Saskatchewan, Canada
> goldie at duke.usask.ca
> 
> 





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