Separating 200-350 kDa protein with PAGE

Peter pxpst2+ at pitt.edu
Thu Nov 11 12:50:04 EST 1999


In article <80f1nj$5s9$1 at nnrp1.deja.com>, Farid Jalali
<fjalali at oci.utoronto.ca> wrote:

> Can anyone suggest a % acrylamide that would be appropriate to separate
> proteins that are in the range of 200-350 kDa using SDS-PAGE. Thanks

5 percent gels should work but they are a pain to deal with.  Very flimsy
to say the least.  With proteins that big, you might be better off using
Gel permeation Chromatography assuming that the dilution will not be a
problem.

regards,
Peter Pediaditakis




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