Hi! Does anybody know a good protocol for inhibiting protein production in fibroblasts (or other TC cells) with cycloheximide? Also, how can I know (w/o using S35-methionine or other radioactively labeled a.a.'s) that the inhibition is happening. Can you see it just by running a whole cell lysate on a gel and staining it with Coomassie blue? Thanks. Irina.