cycloheximide

Irina irka at mit.edu
Thu Nov 11 21:38:59 EST 1999


Hi!
Does anybody know a good protocol for inhibiting protein production in
fibroblasts (or other TC cells) with cycloheximide?
Also, how can I know (w/o using S35-methionine or other radioactively
labeled a.a.'s) that the inhibition is happening.  Can you see it just
by running a whole cell lysate on a gel and staining it with Coomassie
blue?

Thanks.
Irina.





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