Heat inactivation

Bill_A_Nussbaumer at ms.bd.com Bill_A_Nussbaumer at ms.bd.com
Fri Nov 12 11:07:16 EST 1999

Bill A Nussbaumer at BDX
11/12/99 10:49 AM

We routinely boil bacterial DNA just to completely denature it.  The boiled DNA
always then works in amplification reactions.

The heat will probably break down the bacteria though (i.e. lysis).

Bill Nussbaumer

Can anyone tell me if heat inactivation (~100 degrees) will break down
bacteria DNA, especially in the isolation step?
Thanks for helping me.

--For Nature, heartless, witless Nature,
  Will neither know nor cares.--

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