Heat inactivation

Hiranya S. Roychowdhury hroychow at NMSU.EDU
Fri Nov 12 11:30:11 EST 1999

At 09:47 PM 11/12/99 +0800, Chong Wai Yin( Zhang Weixian) wrote:
>Can anyone tell me if heat inactivation (~100 degrees) will break down
>bacteria DNA, especially in the isolation step?
>Thanks for helping me.
>--For Nature, heartless, witless Nature,
>  Will neither know nor cares.--

I guess you are not familiar with "boiling lysis" prep of plasmid DNA.
Boiling does not break down DNA.

Dr. Hiranya Sankar Roychowdhury
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu

More information about the Methods mailing list