Heat inactivation

[Hiranya S. Roychowdhury]

At 09:47 PM 11/12/99 +0800, Chong Wai Yin( Zhang Weixian) wrote:
>Hi!
>Can anyone tell me if heat inactivation (~100 degrees) will break down
>bacteria DNA, especially in the isolation step?
>Thanks for helping me.
>
>Kelvin
>--For Nature, heartless, witless Nature,
>  Will neither know nor cares.--


I guess you are not familiar with "boiling lysis" prep of plasmid DNA.
Boiling does not break down DNA.


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu