general question about PCR

jennnn1970 at my-deja.com jennnn1970 at my-deja.com
Fri Nov 12 21:34:48 EST 1999


Hi all... this is a very basic question about PCR.

I understand the process of collecting tail DNA, making of primers, the
thermalcycles and such. I just don't understand how you'd get a bunch
of fragments to run on a gel at the end of the PCR?

Do you digest the genomic dna first? Do you digest the PCR'd DNA at the
end? Do the primers only extend so far?

I don't understand why the PCR reaction wouldn't copy the WHOLE piece
of DNA.

Confused and can't find any answers in textbooks or the web,

Jen :)

jennnn at hotmail.com


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