general question about PCR

Chris LaRosa plarosa1 at navix.net
Fri Nov 12 23:48:24 EST 1999


Get a piece of paper or a black board and draw it out..........

jennnn1970 at my-deja.com wrote:

> Hi all... this is a very basic question about PCR.
>
> I understand the process of collecting tail DNA, making of primers, the
> thermalcycles and such. I just don't understand how you'd get a bunch
> of fragments to run on a gel at the end of the PCR?
>
> Do you digest the genomic dna first? Do you digest the PCR'd DNA at the
> end? Do the primers only extend so far?
>
> I don't understand why the PCR reaction wouldn't copy the WHOLE piece
> of DNA.
>
> Confused and can't find any answers in textbooks or the web,
>
> Jen :)
>
> jennnn at hotmail.com
>
> Sent via Deja.com http://www.deja.com/
> Before you buy.





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