Multiple amino acid mutagenesis

[mb]

try going to the below site and running the basic homology search,
alignment and prediciton routine.  This should give you a rational basis
for deciding what to do.  Pay close attention to both the structure
prediction and the exposed/buried prediction.  This should give you an
idea of what a given mutation may do.  You can even run the predictions
with potential mutated sequences.  There may also turn out to be
homologous domains of known structure.


http://dodo.cpmc.columbia.edu/predictprotein/


--
Mark Bowen